This product line was developed for general laboratory use. Applied Microspheres can offer many batches of particles with a wide variety of diameters, that can be custom dyed with different fluorescent colours and intensity levels as required. The dyes are incorporated during the polymerisation process, which prevents dye leakage. Applied Microspheres unique technology ensures exceptionally small fluorescence C.V.s. Dispersion in both aqueous media or air are possible without degradation of their fluorescent properties. They are available as aqueous suspensions or as dry powder. The aqueous medium for the suspensions is specially formulated to prevent the formation of agglomerates and for long-term stability. They can be stored at room temperature.
The fluorescent microspheres can be detected with a flow cytometer, epifluorescence microscope, confocal microscope, fluorescence spectrophotometer, amongst others. They can also be identified using a mineral light or black light and can be observed directly in the matrix or media being tested, or they can be collected on membrane filters for examination using the fluorescence microscope. If required, our scientists can modify the polymer microspheres matrix to tune stability in certain solvents. Such particles can be used to study kinetic processes. Targeted colloidal and spectral adjustments of the fluorescent microspheres can mimic complex biological components, such as bacteria, for analytical purpose.
Typical applications include:
Flow cytometry instrument calibration and set-up
Fluorescence microscopy
Fluorescent instrument monitoring
Confocal microscopy reference particles
Filtration media and systems testing
Vial and container cleaning studies
Flow tracing and fluid mechanics
Centrifugation and sedimentation studies
Blue
Excitation wavelength ranging from 380 450 nm.
Emission wavelength ranging from 440 520 nm.
Excitation wavelength ranging from 400 490 nm.
Emission wavelength ranging from 470 560 nm.
Excitation wavelength ranging from 450 570 nm.
Emission wavelength ranging from 550 660 nm.